TOOLS EvaGreen qPCR Mix

  • TOOLS 5X EvaGreen qPCR Mix with ROX

    TOOLS 5X EvaGreen qPCR Mix with ROX

    TOOLS 5X EvaGreen qPCR Mix with ROX is optimized for real-time quantitative PCR assays. The ready-to-load mix includes TOOLS HotTaq DNA polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye and ROX dye according to system requirements. Only water, template and primers need to be added. TOOLS HotTaq DNA polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

  • TOOLS 5X EvaGreen qPCR Mix without ROX

    TOOLS 5X EvaGreen qPCR Mix without ROX

    TOOLS 5X EvaGreen qPCR Mix without ROX is optimized for real-time quantitative PCR assays. The ready-to-load mix includes TOOLS HotTaq DNA polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye. Only water, template and primers needs to be added. TOOLS HotTaq DNA polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

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